Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1993-08-02
1995-10-17
Draper, Garnette D.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 435 691, 435 695, 435 696, 435 694, 435 681, C12N 1567, C12N 1570, C12N 1587
Patent
active
054590515
ABSTRACT:
The invention is directed to recombinant DNA vectors and methods of use thereof. The vectors allow over-expression of proteins in bacterial host cells. The vectors contain a first gene encoding a ubiquitin fusion protein of interest and a second gene encoding a cytoplasmic peptidyl-prolyl cis-trans isomerase gene. Co-expression of the first and second genes allows over-expression of the protein of interest. In some cases the degree of solubility of the protein of interest is also increased.
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Celtrix Pharmaceuticals Inc.
Draper Garnette D.
Fitzgerald David L.
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