Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-11-12
1995-10-17
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 913, 536 2433, C12Q 168, C12P 1934, C12N 1511
Patent
active
054590370
ABSTRACT:
An improved method for the simultaneous sequence-specific identification of mRNAs in a mRNA population allows the visualization of nearly every mRNA expressed by a tissue as a distinct band on a gel whose intensity corresponds roughly to the concentration of the mRNA. In general, the method comprises the formation of cDNA using anchor primers to fix a 3'-endpoint, producing cloned inserts from the cDNA in a vector containing a bacteriophage-specific promoter for subsequent RNA synthesis, generating linearized fragments of the cloned inserts, preparing cRNA, transcribing cDNA from the cRNA using a set of primers, and performing PCR using a 3'-primer whose sequence is derived from the vector and a set of 5'-primers that is derived from the primers used for transcription of cDNA from cRNA. The method can identify changes in expression of mRNA associated with the administration of drugs or with physiological or pathological conditions.
REFERENCES:
Liang et al. (1992), Science 257:967-971.
Liang et al. (1993), Nucl. Acids Res. 21 (14):3269-3275.
White et al. (1989), Trends in Genetics 5(6):185.189.
Stratagene 1993 Product Catalog (Stratagene Cloning Systems), p. 312.
Nadeau, J. H., Bedigian, H. G., Bouchard, G., Denial, T., Kosowsky, M., Norberg, R., Pugh, S., Sargeant, E., Turner, R., and Paigen, B., (1992) Multilocus markers for mouse genome analysis: PCR amplification based on single primers of arbitrary nucleotide sequence. Mammalian Genome 3:55-64.
Woodward, S. R., Sudweeks, J., and Teuscher, C., (1992) Random sequence oligonucleotide primers detect polymorphic DNA products which segregate in inbred strains of mice. Mammalian Genome 3:73-78.
Bishop, J. O., (1974) The Gene Numbers Game. Cell 2:81-86.
Ohta, T. and Kimura, M., (1971) Functional Organization of Genetic Material as a Product of Molecular Evolution. Nature 233:118-119.
Hastie, N. D., and Biship, J. O., (1976) The Expression of Three Abundance Classes of Messenger RNA in Mouse Tissues. Cell 9:761-774.
Bantle, J. A., and Hahn, W. E., (1976) Complexity and Characterization of Polyadenylated RNA in the Mouse Brain. Cell 8:139-150.
Chikaraishi, D. M., (1979) Complexity of Cytoplasmic Polyadenylated and Nonpolyadenylated Rat Brain Ribunucleic Acids. Biochemistry 18(15):3249-3256.
Milner, R. J., and Sutcliffe, J. G., (1983) Gene expression in rat brain. Nucleic Acids Research 11(16):5497-5520.
Sutcliffe, J. G., (1988) mRNA in the Mammalian Central Nervous System. Ann. Rev. Neurosci. 11:157-98.
Adams, M. D., Kelley, J. M., Gocayne, J. D., Dubnick, M., Polymeropoulos, M. H., Xiao, H., Merril, C. R., Wu, A., Olde, B., Moreno, R. F., Kerlavage, A. R., McCombie, W. R., and Venter, J. C., (1991) Complementary DNA Sequencing: Expressed Sequence Tags and Human Genome Project. Science 252:1651-1656.
Adams, M. D., Dubnick, M., Kerlavage, A. R., Moreno, R., Kelley, J. M., Utterback, T. R., Nagle, J. W., Fields, C., and Venter, J. C., (1992) Sequence indentification of 2,375 human brain genes. Nature 355:632-634.
Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., and Tingey, S. V., (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18(22):6531-6535.
Welsh, J., and McClelland, M., (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research 18(24):7213-7218.
Welsh, J., Chada, K., Dalal, S. S., Cheng, R., Ralph, D., and McClelland, M., (1992) Arbitrarily primed PCR fingerprinting of RNA. Nucleic Acids Research 20(19): 4965-4970.
Orita, M., Iwahana, H., Kanazawa, H., Hayashi, K., and Sekiya, T., (1989) Detection of polymorphisms of human DNA by gel electrophoresis and single-strand conformation polymorphisms. Proc. Natl. Acad. Sci. USA 86:2766-2770.
Orita, M., Suzuki, Y., Sekiya, T., and Hayashi, K., (1989) Rapid and Sensitive Detection of Point Mutations and DNA Polymorphisms Using the Polymerase Chain Reaction. Genomics 5:874-879.
Forss-Petter, S., Danielson, P., and Sutcliffe, J. G., (1986) Neuron-Specific Enolase: Complete Structure of Rat mRNA, Multiple Transcriptional Start Sites, and Evidence Suggesting Post-Transcriptional Control. Journal of Neucoscience Research 16:141-156.
Travis, G. H., and Sutcliffe, J. G., (1988) Phenol emulsion-enhanced DNA-driven subtractive cDNA cloning: Isolation of low-abundance monkey cortex-specific mRNAs. Proc. Natl. Acad. Sci. 85:1696-1700.
Erlander Mark G.
Sutcliffe J. Gregor
Carter Philip W.
Fitting Thomas
Schwartz Richard A.
The Scripps Research Institute
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