Reactions using heat-releasable reagents in wax beads

Details Reactions using heat-releasable reagents in wax beads Reactions using heat-releasable reagents in wax beads

1995-03-01

1997-07-01

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 435 71, 435 41, 435 89, 435 914, 435 9141, 435174, 435177, 435182, 436528, 436535, 530812, 530817, 935 16, 935 33, C12P 1934, C12N 1104, C12N 1500, G01N 33544

Patent

active

056437641

ABSTRACT:
A reagent such as a heat resistant enzyme is entrapped in a material such as wax or a liposome that releases the reagent when heated so the reagent is available for reaction. In a preferred embodiment, wax beads containing the reagent are prepared by injecting the reagent into beads of molten wax and cooling to solidify the wax. In another embodiment, droplets of a solution of the reagent are dropped through a layer of molten wax to coat the droplets with the wax and the coated droplets are cooled to solidify the wax. The entrapped reagents have application in nucleic acid hybridizations, polymerase chain reactions (PCR), reverse transcriptase reactions (RTR), nucleic acid sequencing, and product generating reactions such as colorimetic, fluorometric and chemiluminescent enzyme labeled immunoassays.

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