Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Patent
1994-10-21
1997-07-01
Loring, Susan A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
4351722, 435328, 435335, 4353431, 5303873, 53038823, 53038873, C12P 2108, C12N 512, C07K 1600, C07K 1618
Patent
active
056437595
ABSTRACT:
The present invention relates to a method for the selective preparation of hybridoma cell lines which produce a murine monoclonal antibody (MAK) of the IgGl class with a high capacity for including NK cell-relate cytotoxicity against human CD16 antigen by co-culturing the hybridoma cells in the selection medium with unstimulated human NK (natural killer) cells. The invention furthermore relates to a cell line A9 of the DSM deposit number ACC 2148 obtainable in this manner, and a MAK obtainable thereform, and a method for the preparation of bispecific MAK's by the fusion of an anti-CD30 cell line HRS-3 with hybridoma cell lines, which is obtained by the above-mentioned selection process, expecially with the A9 line of DSM deposit number ACC 2148 obtaining especially the bispecific MAK of the HRS-3/A9 cell line with the DSM deposit number ACC 2142. These bispecific MAK's are suitable for the treatment and for the shrinking of established human tumors, especially human Hodgkin's tumors.
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Biotest Pharma GmbH
Loring Susan A.
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