Tissue inhibitor of metalloproteinases

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4353201, 435325, 435348, 435419, 536 235, C12N 1500, C12N 1585, C12N 1563, C12N 1511

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active

056437528

ABSTRACT:
The present invention provides a nucleic acid sequence which encodes a novel tissue inhibitor of metalloproteinases (TIMP-4) which was isolated from cells of human uterus. The invention provides for genetically engineered expression vectors and host cells comprising nucleic acid sequence encoding TIMP-4. The invention also provides for purified TIMP-4.

REFERENCES:
Apte et al., "The Gene Structure of Tissue Inhibitor of Metalloproteinases" (TIMP)-3 and Its Inhibitory Activities Define the Distinct TIMP Gene Family.
Boone TC, et al., "cDNA cloning and expression of a metalloproteinase inhibitor related to tissue inhibitor of metalloproteinases," Proc. Natl. Acad. Sci. USA, 87:2800-2804 Apr. 1990.
Docherty et al., Sequence of human tissue inhibitor of metalloproteinases and its identity to erythroid-potentiating activity, Nature, 318:66-69 (1985).
Freudenstein, mRNA of bovine tissue inhibitor of metalloproteinase: Sequence and expression in bovine ovarian tissue, Biochem Biophys. Res. Comm., 171:250-256 (1990).
Gewert et al., Characterization and expression of a murine gene homologous to human EPA/TIMP: a virus-induced gene in the mounse, EMBO J., 6:651-657 (1987).
Horowitz et al., "Hyperoxic Exposure Alters Gene Expression in the Lung," The Journal of Biological Chemistry, 264(13) 7092-7095 (1989).
Howard et al., "Preferential Inhibition of 72- and 92-kDa Gelatinases by Tissue Inhibitor of Metalloproteinases-2*," The Journal of Biological Chemistry, 266(20) 13070-13075.
Miyazaki et al., "Purification and Characterization of a Two-chain Form of Tissue Inhibitor of Metalloproteinases (TIMP) Type 2 and a Low Molecular Weight TIMP-like Protein," The Journal of Biological Chemistry, 268(19) 14387-14393 (1993).
Pavloff, "A New Inhibitor of Metalloproteinases from Chicken: ChIMP-3" The Journal of Biological Chemistry, 267(24) 17321-17326 (1992).
Stetler-Stevenson, WG, et al. "Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) mRNA Expression in Tumor Cell Lines and Human Tumor Tissues," The Journal of Biological Chemistry, 265(23):13933-13938 Aug. 15, 1990.
Wilde et al., Cloning and Characterization of Human Tissue Inhibitor of Metalloproteinases-3, DNA Cell Biol., 13:711-718 (1994).
Wick et al., "A Novel Member of Human Tissue Inhibitor of Metalloproteinases (TIMP) Gene Family is Regulated during G1 Progression, Mitogenic Stimulation, Differentiation, and Senescence" The Journal of Biological Chemistry, 269(29) 18953-18960 (1994).

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