Substrate useful for separating modified oligonucleotides

Details Substrate useful for separating modified oligonucleotides Substrate useful for separating modified oligonucleotides

1993-03-16

1997-07-01

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

5253294, C12Q 168

Patent

active

056437170

ABSTRACT:
Disclosed is a substrate useful for separating unmodified and modified mononucleotides and oligonucleotides. The substrate includes at least 12% polymer in at least 50% (volume:volume) organic solvent, the organic solvent being a denaturing agent. This substrate is easily removable from the capillary using low pressure. Also provided is a method of separating unmodified and modified mononucleotides and oligonucleotides utilizing this substrate.

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