Immobilization of biologically active substances in a porous sup

Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;...

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435176, 435178, 435182, C12N 1100, C12N 1104, C12N 1114

Patent

active

043236502

ABSTRACT:
Biologically active substances such as enzymes are immobilized in the pores of a porous support material by introducing a solution of the biologically active substance into the pores of the support material, treating the active substance in the pores to temporarily retain the active substance in the pores and immobilizing the active substance in the pores by crosslinking. Temporary retention of the active substance in the pores may be carried out by precipitation with a precipitating agent or by freeze-drying.

REFERENCES:
patent: 3705084 (1972-12-01), Reynolds
patent: 3736231 (1973-05-01), Stanley et al.
patent: 3804719 (1974-04-01), Messing
patent: 3849253 (1974-11-01), Harvey et al.
patent: 3850751 (1974-11-01), Messing
Olson et al., The Use of Tannic Acid and Phenol-Formaldehyde with Glutaraldehyde to Immobilize Enzymes, Proceedings of 166th National Meeting of the American Chemical Society, 1973, Immobilized Enzymes in Food and Microbiological Processes, Plenium Press, N.Y., 8/74, (pp. 54-61).

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