Retroviral vector, a replication system for said vector and avia

Details Retroviral vector, a replication system for said vector and avia Retroviral vector, a replication system for said vector and avia

1998-10-09

2000-08-22

Elliott, George C.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 691, 435440, 435455, 4353201, 435325, 435349, 536 231, C07H 2104, C12N 1511, C12N 1563

Patent

active

061074780

ABSTRACT:
A retroviral vector comprising a retrovirus with infectivity for birds and/or mammals in which at least part of the genomic RNA sequences carrying information for the production of viral proteins required in trans for retroviral replication have been replaced by one or more sequences carrying information to be introduced in a target cell chromosome, characterized in that the primer binding site (PBS) has been modified to a sequence that does not allow strong base pairing with the 3' end or other priming sequence in any naturally occurring tRNA. The present application is directed to the tRNA-like primer.

REFERENCES:
Pedersen, F.S. et al., "Control Points for Retroviral Vector Safety: A Look at the MLV Replication Cycle", abstract of paper presented at Goslar, Germany, conference Sep. 18, 1994.
Barat, C., V. Lullien, O. Schatz, G. Keith, M.T. Nugeyre, F. Gruninger-Leitch, F. Barre-Sinoussi, S.F.J. LeGrice and J.L. Darlix; HIV-1 Reverse Transcriptase Specifically Interacts with the Anticodon Domain of its Cognate Primer t-RNA; The EMBO Journal vol. 8, No. 11, pp. 3279-3285 (1989).
Lund et al., Journal of Virology, Feb. 1997, vol. 71, pp. 1191-1195.
Sang-Moo Kang et al., Journal of Virology, Jan. 1997, vol. 71, No. 1, pp 207-217.
Isel et al., Journal of Biological Chemistry, Dec. 1993, vol. 288, No. 34, pp. 25269-25272.
Wakefield, et al., Journal of Virology, Oct. 1995, vol. 69, No. 10, pp. 6021-6029.
Isel et al., J. Mol. Biol. (1995) 247, pp. 236-250.
Wakefield et al., "Minimal Sequence Requirements of a Functional Human Immunodeficiency Virus type 1 Primer Binding Sequence," J. of Virology vol. 68(3):1605-1614, 1994.
Atze et al. "Reduced Replication of Human Immunodeficiency Type 1 Mutants that Use Reverse Transcriptase Primers Other Than tRNA, .sup.Lys ". J. of Virology vol. 69(5):3090-3097, 1995.
Weiss et al. "Synthetic Human tRNA.sup.Lys and Natural Bovine tRNA.sup.Lys Interact with Human Reverse Transcriptase and Serve as Primers for Retroviral cDNA Synthesis" Gene vol. 111 183-197, 1992.
Lund et al. "Mutated Primer Binding Sites Interacting with Different tRNAs Allow Efficient Murine Leukemia Virus Replication" J of Virology vol. 67(12):7125-7130, 1993.
Kriel et al. The Use of Synthetic tRNA Gene as a Novel Approach to Study in Vivo Transcription and Chromatin Structure in Yeast NAR vol. 19 (14) 3849-3855, 1991.
Barat et al. "Interaction of HIV-1 Reverse Transcriptase with a Synthetic Form of its Replication Primer tRNA.sup.Lys 3". NAR vol. 19(4):751-757 1991.

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