Scanning confocal microscope providing a continuous display

Radiant energy – Photocells; circuits and apparatus – Optical or pre-photocell system

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2504861, 359368, H01J 314

Patent

active

052834336

ABSTRACT:
A scanning confocal microscope scans a sample with an incident beam of radiation, in a raster scan pattern, causing the sample to fluoresce and emit visible radiation in at least two wavelengths. A portion of the fluorescent light retraces a portion of the path optical of incident beam, to a dichroic mirror that separates it from the incident beam for detection by a pair of photomultiplier tubes. A data processor accumulates digital data from the photomultiplier tubes to form a succession of image data frames of the sample being scanned, in the two wavelengths. Image data for a selected number of frames in each wavelength is averaged and then recorded on a single track of a recording system, in an alternating fashion with averaged data for the other wavelength. In addition, a ratio of the averaged data for the two wavelengths is delivered to a video display.

REFERENCES:
patent: 4827125 (1989-05-01), Goldstein
patent: 5149972 (1992-09-01), Faye et al.
"Ophtalmoscope A Balayage Optique," J. Cohen Sabban et al., Horizons de l'Optique 83, 1984, vol. 15, pp. 425-430.

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