Preparation of 1-amino-1-deoxyoligosaccharides and derivatives t

Details Preparation of 1-amino-1-deoxyoligosaccharides and derivatives t Preparation of 1-amino-1-deoxyoligosaccharides and derivatives t

1990-06-05

1994-02-01

Brown, Johnnie R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 111, 536 41, 536124, 536127, 435 84, C07H 100

Patent

active

052833534

ABSTRACT:
The invention resides in oligosaccharide derivatives which are obtained by the enzynmatic cleavage of carbohydrates linked to proteins through asparagine groups. The oligosaccharide moieties of the instant invention are 1-amino-1-deoxyoligosaccharide compounds which are cleaved from a glycoprotein with an aminohydrolase enzyme, wherein the amino group of the oligosaccharide is subsequently substituted with a fluorescent reporter compound. The compounds of the instant invention are characterized by the glycosylamine bonds exhibiting less than 10% hydrolysis at 25.degree. C. in aqueous solution over a two days at pH values between 4-10.

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Moczar, E., et al., Carbohydrate Research 50:133-141 (1976).
"Methods of Enzymology," vol. 83, pp. 263-268, 1982.

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