Holographic based bio-assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

359 1, 359 3, 422 8205, 430 1, 430 2, 435 4, 435 71, 435808, 436501, 436518, 436535, 436805, C12Q 168, G01N 33543

Patent

active

053525825

ABSTRACT:
An apparatus detects a target reactant that binds to an immobilized reactant. The apparatus generates a holographic image at a predetermined location when the reactants are present and bound to one another. The immobilized reactant is bound to a support surface at selected locations. The locations are chosen such that a holographic plate is generated when the target reactant binds to the immobilized reactant. An associated method may be used to detect antibody-antigen reactions, the binding of two strands of nucleic acid, the binding of an enzyme to one of its substrates, and so on.

REFERENCES:
patent: 5166813 (1992-11-01), Metz
Pluddemann, E. P. "Silane Coupling Agents", Plenum, N.Y. 1982.
Partis, M. D., D. G. Griffiths, G. C. Roberts and R. B. Beechey, 1983, Cross-linking of protein by w-maleimido alkanoyl n-hydroxysuccinimido esters. J. Prot. Chemistry, 2, 263.
Kremsky, J. N., J. L. Wooters, J. P. Dougherty, R. E. Meyers, M. Collins and E. L. Brown. 1987. Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5' terminus. Nuc. Acids Research, 15, 2891.
Lund, V., R. Schmid, D. Rickwood, and E. Hornes. 1988. Assessment of methods for covalent binding of nucleic acids to magnetic beads, Dynabeads and the characteristics of the bound nucleic acids in hybridization reactions. Nuc. Acids Research, 16, 10861.
Bhatia, S. K., L. C. Shriver-Lake, K. J. Prior, J. H. Georger, J. M. Calvert, R. Bredehorst and F. S. Ligler. 1989. Use of thiol-terminal silanes and heterobifunctional crosslinkers for immobilization of antibodies on silica surfaces. Anal. Biochem., 178, 408.
Running, J. A. and M. S. Urdea. 1990. A procedure for productive coupling of synthetic oligonucleotides to polystyrene microtiter wells for hybridization capture. Bio Techniques, 8, 276.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Holographic based bio-assay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Holographic based bio-assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Holographic based bio-assay will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-580722

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.