Luciferase assay method

Details Luciferase assay method Luciferase assay method

1990-09-10

1994-02-01

Griffin, Ronald W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

435 6, C12Q 166

Patent

active

052831795

ABSTRACT:
The invention provides improved methods for assaying samples for the presence of a beetle luciferase. The methods of the invention entail improved kinetics of light production from luciferase activity in beetle luciferase-luciferin reactions.

REFERENCES:
patent: 4234681 (1980-11-01), DeLuca-McElroy
patent: 4246340 (1981-01-01), Lundin et al.
patent: 4614712 (1986-09-01), Baldwin et al.
patent: 4833075 (1989-05-01), Vijayalakshmi et al.
Airth et al., "The Function of Coenzyme A in Luminescence" Biochim. et Biophys. Acta., 27 519-532 (1958).
Rhodes and McElroy, "The Synthesis and Function of Luciferyl-Adenylate and Oxyluciferyl-Adenylate," J. Biol. Chem., 233 1528-1537 (1958).
McElroy and Seliger, "The Chemistry of Light Emission," Advances in Emzymol. 25, 119-165 (1963).

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