Methods for using mutant RNA polymerases with reduced discrimina

Details Methods for using mutant RNA polymerases with reduced discrimina Methods for using mutant RNA polymerases with reduced discrimina

1998-06-19

2000-08-22

Fredman, Jeffrey

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 912, 536 221, 536 243, 536 2432, C12Q 168, C12P 1934, C07H 2104

Patent

active

061070378

ABSTRACT:
A method for synthesizing a nucleic acid molecule comprising at least one non-canonical nucleoside triphosphate using a mutant polymerase having a reduced discrimination between canonical and non-canonical substrates is disclosed. The method comprises incubating a template nucleic acid in a reaction mixture comprising the mutant nucleic acid polymerase and the appropriate canonical and non-canonical nucleoside triphosphates which are desired substrates for the mutant nucleic acid polymerase. The present invention is also a method of determining the sequence of a nucleic acid molecule using the mutant polymerase to create a nucleic acid molecule comprising at least one non-canonical nucleoside triphosphate.

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