Method for the direct, exponential amplification and sequencing

Details Method for the direct, exponential amplification and sequencing Method for the direct, exponential amplification and sequencing

1997-12-16

2000-08-22

Sisson, Bradley

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 2433, C12Q 168, C12P 1934, C07H 2104

Patent

active

061070327

ABSTRACT:
A method is described for the direct, exponential amplification and sequencing ("DEXAS") of a DNA molecule from a complex mixture of nucleic acids, wherein truncated DNA molecules as well as DNA molecules of full length are synthesized simultaneously and exponentially between two positions on the said DNA molecule, which initially contains a DNA molecule in a thermocycling reaction, a first primer, a second primer, a reaction buffer, a thermostable DNA polymerase, a thermostable pyrophosphatase (optionally), deoxynucleotides or derivatives thereof and a dideoxynucleotide or derivatives thereof.

REFERENCES:
patent: 4683202 (1987-07-01), Mullis
patent: 4962020 (1990-10-01), Tabor et al.
patent: 5338671 (1994-08-01), Scalice et al.
patent: 5409811 (1995-04-01), Tabor et al.
patent: 5427911 (1995-06-01), Ruano
patent: 5512462 (1996-04-01), Cheng
patent: 5556772 (1996-09-01), Sorge et al.
patent: 5587287 (1996-12-01), Scalice et al.
patent: 5693517 (1997-12-01), Gelfand et al.
patent: 5789168 (1998-08-01), Leushner et al.
patent: 5830657 (1998-11-01), Leushner et al.
International Publication No. WO 96/41014 published Dec. 19, 1996.
International Publicaiton No. WO 96/10640 published Apr. 11, 1996.
International Publication No. WO 94/26766 published Nov. 24, 1994.
Chemical Abstracts, vol. 125, No. 25, 1996, p. 393, 125:319052.
Tabor et al., Proc. Natl. Acad. Sci. USA, vol. 92, pp 6339-6343, Jul. 1995, "A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy-and dideoxyribonucleotides".
Kilger et al., Nucleic Acids Research, vol. 25, No. 10, May 1997, pp. 2032-2034 "Direct DNA sequence determination from total genomic DNA".
International Publication No. WO 93/02212, published Feb. 4, 1993.
Hwang et al., Analytical Biochemistry, vol. 231, No. 2, Nov. 1995, pp. 460-463, "Direct automated sequencing of singl lambda-phage plaques by exponential amplification sequencing".
Sarkar et al., Nucleic Acids Research, 1995, vol. 23, No. 7, pp. 1269-1270, "Semi Exponential cycle sequencing".
Kilger et al., Biol. Chem., vol. 378, pp 99-105, Feb. 1997, "Direct exponential Amplification and Sequencing (DEXAS) of Genomic DNA".
International Publication No. WO 97/42348, published Nov. 13, 1997.
International Publication No. WO 97/40939, published Nov. 6, 1997.
International Publication No. WO 97/23650, published Jul. 3, 1997.
International Publication No. WO 97/41257, published Nov. 6, 1997.
International Publication No. WO 97/41258, published Nov. 6, 1997.
International Publication No. WO 97/41259, published Nov. 6, 1997.
Deng et al., Journal of Microbilogical Methods, vol. 17 (1993) 103-113, "Simultaneous amplification and sequencing of genomic DNA (SAS) . . . ".
Rao, Analytical Biochemistry, vol. 216, 1-14 (1994), "Direct Sequencing of Polymerase Chain Reaction-Amplified DNA".
Chemical Abstracts, vol. 125, 1996, Ref. 319052 v.

Affiliated with

Also associated with

Rating

Method for the direct, exponential amplification and sequencing does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for the direct, exponential amplification and sequencing , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for the direct, exponential amplification and sequencing will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-579001