Liquid purification or separation – Filter – Movable medium
Patent
1997-07-02
2000-08-22
Weber, Jon P.
Liquid purification or separation
Filter
Movable medium
210530, 435221, 435183, 435816, C11D 742, C12S 900, C12N 900, C12N 954
Patent
active
06105786&
ABSTRACT:
A process is disclosed for preparing a detergent powder containing enzymes where the enzymes are extracted from a fermentation broth with a salt and surfactant mixture and directly agglomerated with detergent paste and dried to form the detergent powder. The process results in a two phase system where the enzyme is extracted into the surfactant rich phase and the second phase is salt rich. The process is especially useful for whole or clarified fermentation broths.
REFERENCES:
patent: 4507219 (1985-03-01), Hughes
patent: 4578269 (1986-03-01), Morein
patent: 4728613 (1988-03-01), Brewer et al.
patent: 4992271 (1991-02-01), Fernandes et al.
patent: 5194639 (1993-03-01), Connor et al.
patent: 5254339 (1993-10-01), Morein
patent: 5271840 (1993-12-01), Kresheck
(Item BB) Chemical Abstracts No. 117:107676 (Kitahara, T. et al.) (1991) "Application of Pluronic F68 for aqueous two-phase partition proteins".
Vasudevan, M. et al. (1995) Biotechnology and Bioengineering 46:99-108.
Derwent Abstracts No. 82-04177 (Ferster, M. et al.)(1982) "Application of the two-phase-system aqueous solution Triton X-114 in protein chemistry: extraction of hydrophilic proteins by varying ionic strength and the use of dense sucrose solutions".
Slinde, E. et al. (1976) Eiochimica et Biophysica Acta 445:796-805.
Nikas, Y.J. et al. (1992) 25(18):47974806.
Foster et al. (1982) Hoppe Seyler Z. Physiol. Chem., 363, "Application of the Two-Phase-System Aqueous Solution/Triton X-114 in Protein Chemistry: Extraction of Hydrophilic Proteins by Varying Ionic Strength and the Use of Dense Sucrose Solutions", pp. 1010-1011.
Holm et al. (1986) J. Biol. Chem., 261(33), "Demonstration of the Amphiphilic Character of Hormone-Sensitive Lipase by Temperature-Induced Phase Separation in Tritron X-114 and Charge-Shift Electrophoresis", pp. 15659-15661.
Bordier (1981) J. Biol. Chem., 256(4), "Phase Separation of Integral Membrane Proteins in Triton X-114", pp. 1604-1607.
Terstappen et al. (1992) Biotechnol. Appl. Biochem., 16 (3), "The Use of Detergent-Based Aqueous Two-Phase Systems for the Isolation of Extracellular Proteins: Purification of a Lipase from Pseudomonas cepacia", pp. 228-235.
Terstappen et al. (1993) J. Biotechnol., 28(3), "Protein Partitioning in Detergent-Based Aqueous Two-Phase Systems", pp. 263-275.
Kitahara, T., et al., "Application of Pluronic F68 for Aqueous Two-Phase Extraction of Proteins"J. Chemical Engin. Japan 26(2) :183-188 (1993).
Becker Nathaniel T.
Braunstein Edit L.
Ganshaw Grant C.
Graycar Thomas P.
Anderson Kristen A.
Genencor International Inc.
Gorthey LeeAnn
Horn Margaret A.
Weber Jon P.
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