Process for producing gene products of plasmid having temperatur

Details Process for producing gene products of plasmid having temperatur Process for producing gene products of plasmid having temperatur

1978-05-22

1985-01-22

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4351723, 435 68, 435317, 435820, 935 27, 935 43, 935 60, 935 73, C12N 986, C12N 1500, C12N 100, C12P 2100

Patent

active

044952872

ABSTRACT:
Gene products of plasmid DNA, such as proteins, are prepared in high yields by cultivating bacteria carrying a plasmid which shows a controlled constant plasmid copy number at one temperture and a much higher or totally uncontrolled copy number at a different temperature. The plasmid may be prepared by recombinant DNA technique using a cloning vector showing the temperature dependent plasmid copy number pattern.

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