Chemistry: analytical and immunological testing – Biological cellular material tested
Patent
1994-05-26
1996-10-08
Warden, Jill
Chemistry: analytical and immunological testing
Biological cellular material tested
436 10, 436164, 436172, 436800, 435 2, 435 6, 435 29, 435 34, G01N 3348
Patent
active
055630707
ABSTRACT:
The invention provides a method of quantitating reticulocytes with high sensitivity and high accuracy at low cost employing a fluorescent dye suitable for the staining of reticulocytes. A blood sample stained with a fluorescent dye composition containing TOTO-3, TO-PRO-3, POPO-3, PO-PRO-3, YOYO-1, YO-PRO-1, YOYO-3 or YO-PRO-3 as the fluorescent dye is exposed to light in the red-wave length region in the case of TOTO-3 or TO-PRO-3, light in the green region for POPO-3 or PO-PRO-3, light in the blue region for YOYO-1 or YO-PRO-1, or light in the orange region for YOYO-3 or YO-PRO-3 and the resultant fluorescent emission from the blood sample is measured to enumerate reticulocytes.
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Hirako Sinichi
Inagami Misako
Tokita Muneo
Yamamoto Kohji
Omron Corporation
Wallenhorst Maureen M.
Warden Jill
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