Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1995-05-26
1996-10-08
Benzion, Gary
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352404, 43524048, 43524049, 43524054, 800200, 800DIG49, 800DIG50, 800DIG51, 47 58, A01H 400, A01H 700, C12N 504
Patent
active
055630618
ABSTRACT:
The invention is a method for reproducing coniferous trees by somatic embryogenesis using plant tissue culture techniques in a multistage culturing process. A suitable explant, typically the fertilized embryo excised from an immature seed, is first cultured on a medium that induces multiple early stage proembryos. These are multiplied in a second culture having reduced growth hormones. Maltose is supplied as the carbon and energy source in the second culture. Alternatively, a sucrose-containing maintenance and multiplication medium may be used followed by at least two subcultures on a maltose based maintenance medium. The early stage embryos grow in size and vigor to advanced early stage embryos. The embryos are then transferred to a cotyledonary embryo development culture. After several weeks somatic embryos having the appearance of zygotic embryos will have formed. These may be germinated before or after storage and transplanted to soil for further growth. Maltose used in the maintenance and multiplication culture results in larger and more robust advanced early stage embryos which, in turn, produce cotyledonary embryos very similar in morphology to natural zygotic embryos. The use of maltose at earlier stages of embryo development is more important than its use for embryo maturation.
REFERENCES:
patent: 4801545 (1989-01-01), Stuart et al.
patent: 5036007 (1991-07-01), Gupta et al.
patent: 5187092 (1993-02-01), Uddin
patent: 5236841 (1993-08-01), Gupta et al.
patent: 5413930 (1995-05-01), Becwar et al.
Nagmani, R. and R. J. Dinus. Paper at 21st Southern Tree Improvement Conference, Knoxville, TN, Jun. 17-20, 1991.
Schuller, Astrid and Gerhard Reuther. Plant Cell Reports 132: 199-202 (1993).
Strickland, Steven G., James W. Nichol, Carol M. McCall, and David A. Stuart. Plant Science 48: 113-121 (1987).
Tremblay, Laurence and Francine M. Tremblay. Plant Cell, Tissue and Organ Culture 27: 95-103 (1991).
Benzion Gary
Weyerhaeuser Company
LandOfFree
Method for reproducing conifers by somatic embryogenesis using a does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for reproducing conifers by somatic embryogenesis using a, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for reproducing conifers by somatic embryogenesis using a will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-56456