Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...
Patent
1994-10-31
1996-10-08
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Stablizing an enzyme by forming a mixture, an adduct or a...
530412, 530427, C12N 996, C07K 114
Patent
active
055630570
ABSTRACT:
A method for refolding an enzyme from a misfolded configuration to a second native and active configuration is presented. The method comprises adding a linear alkyl detergent to a misfolded enzyme to form an enzyme-detergent complex. Then the enzyme-detergent complex is contacted with a cyclodextrin to allow the enzyme to assume a second active configuration. The misfolded enzyme may have been previously denatured by heat or by chemical means other than a detergent.
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Gellman Samuel H.
Rozema David B.
Naff David M.
Weber Jon P.
Wisconsin Alumni Research Foundation
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