Homogeneous method for assay of double-stranded nucleic acids us

Details Homogeneous method for assay of double-stranded nucleic acids us Homogeneous method for assay of double-stranded nucleic acids us

1995-12-21

1996-10-08

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 435810, 536 231, 536 2433, 536 2532, 935 8, 935 76, 935 77, 935 78, 546152, 546176, C12Q 168, C12P 1934, C07H 2102, C12N 1500

Patent

active

055630375

ABSTRACT:
A highly sensitive homogeneous assay allows for quantitative detection of amplified nucleic acids. This detection is achieved during or after amplification with a high affinity fluorescent dye which is from the class of unsymmetrical cyanine dyes having at least two positive charges and a binding constant (K.sub.b) within the range of from about 1.times.10.sup.4 to about 5.times.10.sup.5 (molar.sup.-1). The reagents used for the assay can be contained in a kit designed for amplification such as by polymerase chain reaction.

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