Methods for the detection of non-pathogenic HIV-1 strains contai

Details Methods for the detection of non-pathogenic HIV-1 strains contai Methods for the detection of non-pathogenic HIV-1 strains contai

1995-06-07

2000-01-18

Adams, Donald E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 71, 435 6, C12Q 170, C12Q 168, G01N 3353

Patent

active

060156619

ABSTRACT:
The present invention is directed toward immunologic- and nucleic acid-based methodologies for the detection of non-pathogenic human immunodeficiency virus type 1 (HIV-1) strains in the body fluids of HIV-infected individuals. A blood donor infected with HIV-1 and a cohort of six blood or blood product recipients infected from this donor were studied. These patients, who remained free of HIV-1-related disease and displayed stable and normal CD4 lymphocyte counts 10 to 14 years after infection, were termed long-term nonprogressors (LTNPs). The molecular characterization of HIV-1 sequences obtained from either virus isolates or patient peripheral blood mononuclear cells (PBMCs) of LTNPs identified similar deletions in the nef gene and in the region of overlap of nef and the U3 region of the long terminal repeat (LTR). These deletions corresponded to amino acids 166-206, or nucleotides 9281 to 9437, of the HIV-1.sub.NL43 nef/LTR region. Methods were developed to detect the presence of nonpathogenic HIV-1 strains carrying these deletions in HIV-infected patients.

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