3'-RNA labelling with terminal transferase

Details 3'-RNA labelling with terminal transferase 3'-RNA labelling with terminal transferase

1995-02-28

1996-11-12

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 911, 435 912, 536 243, 536 2431, 536 2432, C12Q 168, C12Q 170, C12P 1934, C07H 2104

Patent

active

055739130

ABSTRACT:
The present invention concerns a process for introducing non-radioactively labelled deoxynucleotides into nucleic acids and RNA molecules which at their 3' end contain at least one deoxynucleotide which carries a non-radioactive marker group.

REFERENCES:
patent: 4591564 (1986-05-01), Watson
Boehringer Mannheim Biochemia, Catalog; "Biochemicals for Molecular Biology" and Boehringer Mannhiem; Colloquium Molecular Biology.
Feds Letters, vol. 18, No. 2, Nov. 1, 1971, pp. 280-282, "Oligoribonucleotides as primer for terminal deoxynucleotidyl transferase".
Biochem. and Biophys. Res. Communications, vol. 46, III, "Enzymatic synthesis of polynucleotides covalently linked to an oligoribonucleotide primer". pp. 2141-2147, 1972.
Anal. Biochem. (1991), vol. 192, No. 1, Jan. 1991, "Nonradioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing with terminal transferase". pp. 222-231.
Anal. Biochem. (1995), vol. 224, No. 1, Jan. 1995, "Nonradioactive 3'-end-labeling of RNA molecules of different lengths by terminal deoxynucleotidyltransferase". pp. 446-449.
Duck et al, BioTechniques 9(2) 142-145, 1990.
Guitierrez et al, The Lancet, 339:715-721, 1992.
James, W. Antiviral Chemistry and Chemotheraphy 2(4)199-214, 1991.

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