DNA sequence corresponding to the minimal essential promoter of

Details DNA sequence corresponding to the minimal essential promoter of DNA sequence corresponding to the minimal essential promoter of

1998-01-13

2000-01-18

Jones, W. Gary

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 243, 435 6, C07H 2102, C07H 2104, C12Q 168

Patent

active

060153768

ABSTRACT:
Elucidation of the regulation of human sodium-iodide symporter (hNIS) gene expression is critical to understanding its effects on iodide concentration abilities of thyroid and thyroid carcinomas. A 1.2 kb portion of the 5'-flanking region of the hNIS gene has been isolated and characterized, and the nucleotide sequence for the minimal essential promoter of the hNIS gene is shown in FIG. 1. Transient transfections with chimeric luciferase-reporter constructs into a differentiated human thyroid cell line, KAT-50, as well as non-thyroidal cells, define an active promoter having tissue-specificity. KAT-50 cells expressed hNIS mRNA and were capable of thyrotropin-responsive iodide uptake in vitro.

REFERENCES:
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Cloning of the Human Sodium Iodide Symporter, P.A. Smanik, Q. Liu, T.L. Furminger, K. Ryu, S. Xing, E.L. Mazzaferri, S.M. Jhiang, Biochemical and Biophysical Research Communications, Article No. 1358, pp. 226, 339-345 (1996).
Cloning and Characterization of the Thyroid Iodide Transporter, G. Dal, O. Levy, N. Carrasco, Nature, vol. 379, pp. 458-459, Feb. 1, 1996.
Regulation by Thyroid-Stimulating Hormone of Sodium/Iodide Symporter Gene Expression and Protein Levels in FRTL-5 Cells, T. Kogai, T. Endo, T. Saito, A. Miyazaki, A. Kawaguchi, T. Onaya, Endocrinology, vol. 138, No. 6, pp. 2227-2232 (1997).
Expression, Exon-Intron Organization, and Chromosome Mapping of the Human Sodium Iodide Symporter, P.A. Smanik, K. Ryu, K.S. Theil, E.L. Mazzaferri, S.M. Jhiang, Endocrinology, vol. 138, No. 8, pp. 3555-3558 (1997).
Promoter Characterization of the Rat Na.sup.+ /1.sup.- Symporter Gene, Biochemical and Biophysical Research Communications, Article No. RC977432, Q. Tong, K. Ryu, S.M. Jhiang, pp. 239, 34-41 (1997).
Thyroid Transcription Factor-1 Activates the Promoter Activity of Rat Thryroid Na.sup.+ /1.sup.- Symporter Gene, T. Endo, M. Kaneshige, M. Nakazato, M. Ohmori, N. Harii, T. Onaya, Molecular Endocrinology (1997), vol. 11, pp. 1747-1755.

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