Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1989-04-25
1992-01-14
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
43524023, 435 2, 435267, 424577, C12N 508, A61K 3528
Patent
active
050810308
ABSTRACT:
The present invention comprises a process for release from the cell-receptor complex of positively selected cells in viable, functional condition, where a ligand involved in the particular receptor-ligand interaction utilized for the affinity purification is selectively attacked by one or more degradative enzymes specific for that ligand. A resulting cell suspension can be obtained substantially free of receptor material.
This invention, in one embodiment, contemplates a method for positive stem cell selection, utilizing anti-MY10 and immunomagnetic microspheres to isolate CD34-positive marrow cells and employing an enzyme to release micropheres from the isolated CD34-positive cells. Reproducible enzymatic cleaving of immunomagnetic microspheres from MY10-positive cells can be achieved by brief treatment of the preparation with papain or chymopapain. The isolated CD34-positive cells are particularly desirable for bone marrow transplantation.
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The U.S. Government has a paid up license in this invention and the right in limited circumstance to require the patent owner to license others on reasonable terms as provided for by the terms of Grant No. R01 CA 32318 awarded by the National Institutes of Health.
Elliott George C.
The Johns Hopkins University
Weimar Elizabeth C.
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