Method for measuring the amount of polyamines in a biological sa

Details Method for measuring the amount of polyamines in a biological sa Method for measuring the amount of polyamines in a biological sa

1988-12-28

1992-01-14

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 26, 435810, 436 64, 436175, 436813, 436815, C12Q 132, C12Q 126

Patent

active

050810162

ABSTRACT:
A method for measuring the amount of polyamines in a biological sample is provided. The method comprises utilizing diamine oxidase and/or polyamine oxidase, aminoalkylaldehyde dehydrogenase and NAD.sup.+ or NADPH.sup.+, wherein the improvement comprises carrying out the method in the presence of an acidic aromatic compound.

REFERENCES:
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Chemical Abstracts, vol. 110, No. 19, May 8, 1989, p. 44, Abstract No. 169814u, Columbus, Ohio, U.S.; H. Watanabe et al.: "Reducing substance removal from biological samples in optical analysis".
Methods of Enzymic Analysis, vol. 8, 1985, 3rd edition, pp. 566-572, Verlag-Chemie, DE.; S. Kubota et al.: "Polyamines".
Production of Extracellular Polyamine Oxidase by Penicillium sp. No. PO-1, Yoshinori Kobayashi and Koki Horikoshi, (1981).
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Biochimica Biophysica Acta, 705 (1982), pp. 133-138.
Biochimica Biophysica Acta, 743 (1983), pp. 431-436.
Agric. Biol. Chem., 50(8) (1986), pp. 2009-2016.
Nippon Nogeikagaku Kaishi, vol. 62, No. 3 (Mar. 15, 1988), pp. 131, 417.
Japanese Journal of Clinical Laboratory Automation, vol. 13, No. 4 (Aug. 1, 1989), pp. 410-412.

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