Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1997-01-22
2000-07-11
Spector, Lorraine
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 6, 435 72, 435 8, 435 691, 435325, 435365, 4352523, 43525411, 536 2372, 536 241, 536 231, 530350, G01N 33566, C12N 1539, C12N 510, C07K 1403
Patent
active
060871156
ABSTRACT:
The present invention is directed to a constitutively active G protein coupled receptor of human herpesvirus 8, as well as a method of identifying negative antagonists of a constitutively active G protein coupled receptor. The method comprises co-expressing in a host cell a constitutively active G protein coupled receptor and a reporter protein, wherein expression of the reporter protein is controlled by a promoter responsive to a signalling pathway activated by the constitutively active G protein coupled receptor; exposing the host cell to a test substance; and determining a level of reporter protein activity, wherein the level of reporter protein activity indicates effectiveness of the test substance as a negative antagonist of the constitutively active G protein coupled receptor. The invention further provides a method of preventing tumor formation or cell proliferation caused by a constitutively active G protein coupled receptor. This method comprises administering an amount of the negative antagonist so identified to a subject in an amount effective to prevent tumor formation or cell proliferation.
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Arvanitakis Leandros
Cesarman Ethel
Geras-Raaka Elizabeth
Gershengorn Marvin C.
Cornell Research Foundation Inc.
Kaufman Claire M.
Spector Lorraine
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