Antisense oligonucleotide inhibition of the RAS gene

Details Antisense oligonucleotide inhibition of the RAS gene Antisense oligonucleotide inhibition of the RAS gene

1994-08-26

1996-11-19

Elliott, George C.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

536 245, 935 34, C12N 506, C07H 2104

Patent

active

055762085

ABSTRACT:
Compositions and methods are provided for the modulation of expression of the human ras gene in both the normal and activated forms. Oligonucleotides are provided which are specifically hybridizable with RNA or DNA deriving from the human ras gene, having nucleotide units sufficient in identity and number to effect such specific hybridization. Oligonucleotides specifically hybridizable with a translation initiation site or with the codon-12 mutation of activated ras are provided. Such oligonucleotides can be used for diagnostics as well as for research purposes. Methods are also disclosed for modulating ras gene expression in cells and tissues using the oligonucleotides provided, and for specific modulation of expression of the activated ras gene. Methods for diagnosis, detection and treatment of conditions arising from the activation of the H-ras gene are also disclosed.

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