Method for purifying factor VIII:C, von Willebrand factor and co

Details Method for purifying factor VIII:C, von Willebrand factor and co Method for purifying factor VIII:C, von Willebrand factor and co

1988-03-02

1993-04-06

Wax, Robert A.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Blood proteins or globulins, e.g., proteoglycans, platelet...

530413, C07K 318, C07K 320

Patent

active

052005100

ABSTRACT:
Methods for purifying factor VIII:C, von Willebrand factor (vWF) or complexes thereof from heterogeneous biological fluids are disclosed. The methods utilize a binding peptide, specific to either factor VIII:C or vWF, bound to an insoluble matrix. Peptides suitable for use within the methods are also disclosed.

REFERENCES:
patent: Re32011 (1985-10-01), Zimmerman et al.
Honda et al. 1986, J. Biol. Chem. 261(27):12579-12585.
Wicki et al. 1985, Eur. J. Biochem. 153:1-11.
Wilchek et al. 1984, Methods in Enzymology, 104:3-55.
Cuatrecasas et al 1971, Ann. Rev. Biochem. 40:259-278.

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