Method for counting overlapping cell populations in a distributi

Details Method for counting overlapping cell populations in a distributi Method for counting overlapping cell populations in a distributi

1981-11-12

1984-10-02

Martin, John C.

Image analysis

Histogram processing

For setting a threshold

377 10, G06K 900

Patent

active

044752369

ABSTRACT:
A sample having a mixture of unknown stained cells and known cells having different staining characteristics is analyzed rapidly, a cell at a time, in a flow cytometry system having a sample stream dimension in the range of expected cell dimensions. The cells are illuminated with focused illumination and fluorescence is detected and related to the number of cells. The resulting histogram of the mixture sample may be analyzed by counting the cells in a controlled population below a relatively low threshold value of fluorescence intensity to form a first fraction and relating this fraction to the fraction of cells in the sample mixture below the threshold value of fluorescence intensity to determine the number of unknown cells in the sample mixture.

REFERENCES:
patent: 4071891 (1978-01-01), Barrows
patent: 4284355 (1981-08-01), Hansen et al.
patent: 4295199 (1981-10-01), Curry et al.
patent: 4325706 (1982-04-01), Gershman et al.
patent: 4330745 (1982-05-01), Hayashi
patent: 4336029 (1982-06-01), Natale

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