Homogeneous chemiluminescent specific binding assay

Details Homogeneous chemiluminescent specific binding assay Homogeneous chemiluminescent specific binding assay

1979-06-21

1983-05-10

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

422 61, 436536, 436805, 436808, 436817, G01N 3354, G01N 3358, G01N 2176

Patent

active

043830315

ABSTRACT:
A homogeneous specific binding assay which employs a substance having reactant activity, i.e., a reactant, in a chemiluminescent reaction as a labeling substance in the detection of a ligand in a liquid medium. The assay employs a conjugate formed of a specific binding substance coupled to the chemiluminescent reactant. The activity of the conjugated reactant as a constituent of the chemiluminescent reaction is affected by reaction between the specific binding substance in the conjugate and a specific binding counterpart thereto. The presence of a ligand in a liquid medium may be determined using competitive or displacement binding or sequential saturation techniques wherein the specific binding substance in the conjugate is the ligand or a specific binding analog thereof, or using a direct binding technique wherein the specific binding substance is a specific binding partner of the ligand. The effect of the specific binding reaction on the chemiluminescent activity of the conjugated reactant is related to the presence or amount of the ligand in the liquid medium tested.

REFERENCES:
patent: 3935074 (1976-01-01), Rubenstein
patent: 3998943 (1976-12-01), Ullman
patent: 4011219 (1977-03-01), Nishii
patent: 4104029 (1978-08-01), Maier
patent: 4181650 (1980-01-01), Maier
patent: 4225485 (1980-09-01), Buckler
patent: 4238195 (1980-12-01), Boguslaski
patent: 4318980 (1982-03-01), Boguslaski

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