Polysaccharide matrices for use as adsorbents in affinity chroma

Details Polysaccharide matrices for use as adsorbents in affinity chroma Polysaccharide matrices for use as adsorbents in affinity chroma

1974-05-31

1976-03-30

Czaja, Donald E.

Liquid purification or separation

With alarm, indicator, register, recorder, signal or...

Responsive to fluid flow

195 63, 195 68, 195DIG11, 210502, 260 9, 260112R, 260121, 260122, 260212, 260209R, 260209D, 2602333R, 260234D, B01D 1508, C07G 700, C07H 100

Patent

active

039473520

ABSTRACT:
Polysaccharide matrices, e.g., agarose, for use in certain affinity chromatography procedures, are activated with sodium metaperiodate, derivatized with a symmetrical dihydrazide and thence reductively stabilized, preferably with sodium cyanoborohydride. Moreover, intermediate Schiff bases formed from the NaIO.sub.4 -oxidized polysaccharide are selectively, reductively stabilized with said sodium cyanoborohydride.

REFERENCES:
patent: 3850798 (1974-11-01), Sjoquist
Abstracts of Papers, Amer. Chem. Soc., 166th National Meeting, (Chicago, Ill. 1973), "Affinity Chromatography," Parikh et al.

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