Mutant luciferase of a firefly

Details Mutant luciferase of a firefly Mutant luciferase of a firefly

1993-06-15

1994-07-19

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435 712, 4351723, 4352523, 43525233, 4353201, 935 10, 935 14, 935 27, 935 56, 536 232, C12N 902, C12N 1553, C12N 1570

Patent

active

053309065

ABSTRACT:
The present invention provides industrially useful luciferase. Mutant luciferase of the invention is produced by culturing a microorganism belonging to the genus Escherichia which harbors a recombinant DNA containing the mutant luciferase gene of a firefly. Mutant luciferase can produce red, orange or green color of light which can not be produced by wild type luciferase. Mutant luciferase can be used to measure ATP accurately in a colored solution such as red (e.g., blood), orange, or green in which wild-type luciferase has not provided reliable results.

REFERENCES:
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Chu et al. Virology 98:161-181 (1979).
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Wood et al., 1989, Science, 244:700-702.
Wood et al., 1989, J. Bioluminescence and Chemiluminescence 4:289-301.
Wood et al., 1989, J. Bioluminescence and Chemiluminescence 4:31-39.
Wood, J. Bioluminescence and Chemiluminescence 5:107-114 (Paper associated with talk presented at: Symposium on Lux Genes: Basic, Applications and Commercial Prospects, Cambridge, U.K., Dec., 1989).

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