Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-05-16
1999-12-21
Minnifield, Nita
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 72, 435 732, 435 735, 435 73, 435 79, 435 29, 435261, 435 34, 435174, 436518, 436524, G01N 3353, G01N 33543, G01N 33537, G01N 33569
Patent
active
060047666
ABSTRACT:
A method is provided for the detection of low levels of a microorganism in a sample in the presence of competing microflora, comprising the steps of: (a) exposing the sample to a solid support to which are adsorbed antibodies specific for said microorganism; (b) washing the solid support to remove unbound material; (c) combining the solid support with sterile nutrient broth which permits replication of said microorganism; (d) incubating the solid support with said nutrient broth at a temperature and for a time sufficient to allow said microorganism to replicate and be recaptured by said antibodies on said solid support; (e) releasing the bound microorganism from the solid support into a solution; and (f)assaying the solution for the microorganism. A kit for performing the assay also is provided. This kit comprises a solid support to which are adsorbed antibodies specific for the microorganism, a first container comprising a washing solution, a second container comprising a sterile nutrient broth, and, optionally, a third container comprising a solution comprising a releasing agent.
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Ash Megan
Atrache Vincent H.
Van Huynh Ca
Biotechnology Australia Pty Limited
Minnifield Nita
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