Purification method for materials having nick-translation abilit

Details Purification method for materials having nick-translation abilit Purification method for materials having nick-translation abilit

1993-04-30

1995-07-11

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435815, 435816, C12N 912

Patent

active

054320720

ABSTRACT:
The present invention pertains to a method of purification of DNA polymerase I, and the polymerase and Nick-translation activities thereof. In one embodiment, the method of purification is directed to circumstances where there are amplified amounts of the same relative to that which is found naturally occurring. In another embodiment, the purification method is directed to shortening the time period for the purification of the same whether in an amplified amount or not as compared to the time taught in the prior art.

REFERENCES:
patent: 5234829 (1993-08-01), Brown
Kelley, W 9., et. al. (1979) J. Biol Chem. 254 (9), 3206-3210.
Kitayam, S., et. al. (1977) BioChim. Biophys Acta 475, 23-31.
Bekkering-Kaylaars, S.A.M., et al. (1972) BioChim. Biophys. Acta 272, 526-538.
Loeb, LA in "The Enzymes", 3rd Ed, P.O. Boyer, ed. Academie Press, N.Y. 1972, pp. 173-189.
Freifelder, P. (1982) Physical Biochemistry, 2nd Ed., W. H. Freeman 2nd Co., pp. 270-272.

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