Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering
Patent
1975-06-23
1977-07-12
Prescott, Arthur C.
Chemistry: electrical and wave energy
Apparatus
Coating, forming or etching by sputtering
204180G, G01N 2726
Patent
active
040353770
ABSTRACT:
Apparatus for vertical slab gel electrophoresis has upper and lower tanks having open tops. The tanks are supported in stepped relation to each other by a vertical support plate which forms a front wall of one tank and a rear wall of the other. At least part of the wall of the upper tank formed by the support plate has a top edge which is lower than the other top edges of the tank. A substantially open-ended chamber is formed by clamping a plastic plate to a glass plate with strip members between side edges of the plates. The plastic plate is held against the spport plate for reinforcing the rigidity of the plastic plate with the support plate. A portion of the top edge of the plastic plate is lower than the top edge of the glass plate and intermediate the lower edge portion of the wall of the upper tank formed by the support plate and the other top edges of the upper tank. Partitions extend across the chamber from the plastic plate to the glass plate and down into the chamber from the top, lower edge of the plastic plate at least to a slab of gel which fills the chamber to form wells over the gel for receiving samples. An electrolytic buffer solution is placed in both tanks, the solution in the upper tank flowing over the lower edges of the support plate and plastic plate to the glass plate to cover the samples in the wells. Opposite electric potentials of the appropriate polarity are then applied to electrodes in both tanks to cause the samples to migrate electrophoretically into the gel, the position of the samples in the gel then being an indication of the composition of the samples.
REFERENCES:
patent: 3129158 (1964-04-01), Raymond et al.
patent: 3719580 (1973-03-01), Roberts et al.
patent: 3822197 (1974-07-01), Nees et al.
patent: 3856655 (1974-12-01), Roberts
patent: 3879280 (1975-04-01), Peterson et al.
patent: 3932265 (1976-01-01), Hoefer
Cold Spring Harbor Laboratory
Prescott Arthur C.
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