Method of high speed scintillation autoradiography

Details Method of high speed scintillation autoradiography Method of high speed scintillation autoradiography

1975-10-30

1977-07-12

Wolk, Morris O.

Chemistry: physical processes

Physical processes

Crystallization

23230B, 1951035R, 250304, 424 1, 424 15, G01N 2300, G01N 2304

Patent

active

040351556

ABSTRACT:
Autoradiography using high specific activity tritiated thymidine (40-60 curies/millimole) and nuclear track emulsion impregnated with a liquid scintillator provides a very rapid method to determine labelling index and growth fraction of cell suspensions. Low temperature (-20.degree. to -85.degree. C) emulsion exposure for 20 - 60 minutes is sufficient for excellent labelling, thus allowing rapid clinical decision making. Exposure times for research techniques requiring very low energy labelling can be considerably shortened, e.g., from 6 months to 2 weeks or less. This technique therefore has broad applicability to biology and medicine.

REFERENCES:
chemical Abstracts, 68: 833c (1968).
Chemical Abstracts, 69: 83361s-t (1968).
Chemical Abstracts, 79: 112082r (1973).
Chemical Abstracts, 81: 74375v (1974).

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