Clinical procedure for measuring lipoprotein free cholesterols

Details Clinical procedure for measuring lipoprotein free cholesterols Clinical procedure for measuring lipoprotein free cholesterols

1978-07-26

1979-09-11

Prescott, Arthur C.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204180G, 204299R, 424 12, 23230B, G01N 2726, G01N 3316

Patent

active

041674677

ABSTRACT:
An electrophoresis method of determining the concentration of high density lipoprotein (HDL) free cholesterols in body fluids and simultaneously determining the concentration of very low density lipoprotein (VLDL) and low density lipoprotein (LDL) free cholesterols in the fluid. The method includes applying a small sample of the fluid to an electrophoresis support medium, applying a direct current across the medium, applying a developing substrate to the electrophoresed lipoproteins and quantitatively determining the concentration of each lipoprotein free cholesterol. This method does permit direct and simultaneous measurement of each lipoprotein free cholesterol fraction while eliminating precipitation of each fraction as required by the prior art.

REFERENCES:
patent: 3808118 (1974-04-01), Golias
patent: 3873433 (1975-03-01), Seidel et al.
patent: 4041021 (1977-08-01), Bohn
patent: 4094759 (1978-06-01), Ruhenstroth-Bauer et al.

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