Competitive ELISA for the detection of HTLV-III antibodies

Details Competitive ELISA for the detection of HTLV-III antibodies Competitive ELISA for the detection of HTLV-III antibodies

1985-05-24

1987-04-28

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 5, 435810, 436518, 436528, 436543, 436808, 436809, 436811, 436825, 935110, G01N 3353

Patent

active

046614451

ABSTRACT:
A competitive enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies is disclosed. This ELISA technique is more sensitive, more specific, and more accurate than known ELISA techniques. The competitive ELISA of this invention is particularly suited to the detection of human T-cell leukemia-lymphoma virus type III (HTLV-III). Antigenic or viral fragments are bound to a solid support. The bound fragments are then incubated with a heterologous antiserum which binds to the bound fragments. Test sera is then added to the bound fragments and tested for absorbance.

REFERENCES:
patent: 4520113 (1985-05-01), Gallo
Chemical Abstracts 103: 118450v, Casey (1985).
Kalyanaraman, Virology, 32, pp. 61-70 (1984).
Wisdom, Clinical Chemistry, 22(8), pp. 1243-1244 (1976).
Yolken, Reviews of Infectious Diseases, 4(1), pp. 35, 47, 48, 49 (1982).

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