Homogeneous Immunoassay process using covalent conjugate of enzy

Details Homogeneous Immunoassay process using covalent conjugate of enzy Homogeneous Immunoassay process using covalent conjugate of enzy

1993-07-14

1995-09-05

Spiegel, Carol A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 7930, 435 7940, 435 7950, 435174, 435 14, 435 18, 435 22, 435964, 435969, 435970, 435972, 436537, 436538, 5303881, 5303911, 530866, G01N 3353

Patent

active

054478464

ABSTRACT:
An enzyme-labelled antibody adapted for use in a homogeneous immunoassay is provided. The enzyme-labelled antibody is a conjugate of an enzyme with two or more different monoclonal antibodies, each of the monoclonal antibodies being capable of specifically recognizing and binding to a different epitope of the same antigen. By using the enzyme-labelled antibody in the homogeneous enzyme immunoassay process, an analyte can be quantitatively analyzed at a higher sensitivity through a simple operation. Also provided is a dry immunoassay element comprising an immunological reaction layer containing the enzyme-labelled antibody. By the provision of such an immunoassay element, a further simplified quick analysis of an analyte is realized to give an accurate result.

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