Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1992-12-30
1995-09-05
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 935 77, 935 78, C12Q 168, C12P 1934
Patent
active
054478448
ABSTRACT:
A diagnostic assay is provided for identifying closely-related strains or species of bacteria which possess common insertion sequence which is present in different positions within the genomic or plasmidic DNA for the different strains or species. A fragment of DNA defined by a site within the insertion sequence and another site outside the insertion sequence is amplified, such as by polymerase chain reaction. By appropriately preselecting a different size fragment to be amplified in each of the candidate organisms, those organisms which are actually present in a biological sample can be positively identified by size of the amplified fragments.
REFERENCES:
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P. H. Vary et al., "Use of Highly Specific DNA Probes and the Polymerase Chain Reaction to Detect Mycobacterium paratuberculosis in Johne's Disease," J. Clin. Microbiol. 28(5): 933-937 (May 1990).
Shirley M. Halling et al., "DNA Polymorphism and Repetitive DNA in Brucella," Abstract, 42nd Annual Brucellosis Research Conference, Nov. 4-5, 1989.
Shirley M. Halling et al., "A Highly Repeated DNA Sequence and DNA Polymorphism in Brucella ovis, " Abstract H-248, 90th Annual Meeting of American Society for Microbiology, May 13-17, 1990, Anaheim, Calif.
Shirley M. Halling et al., "Polymorphism in Brucella spp. Due to Highly Repeated DNA," J. Bacteriol. 172(12): 6637-6640 (Dec. 1990).
Fred M. Tatum et al., "Nucleotide Sequence of an Insertional Element in Brucella ovis," Abstract H-36, 90th Annual Meeting of American Society for Microbiology, May 13-17, 1990, Anaheim, Calif.
Betsy J. Bricker et al., "Analysis of a Repeated DNA Element in Brucella Species," Abstract H-247, 90th Annual Meeting of American Society for Microbiology, May 13-17, 1990, Anaheim, Calif.
Bricker Betsy J.
Halling Shirley M.
Fado John D.
Ribando Curtis P.
Silverstein M. Howard
The United States of America as represented by the Secretary of
Zitomer Stephanie W.
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