Method for the selective detection of microbial nucleotides

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase

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435 18, 435 29, 435 34, C12Q 148, C12Q 134, C12Q 102, C12Q 104

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049065653

ABSTRACT:
A method for the selective determination of microbial cells, in a sample suspected of containing both microbial and non-microbial cells, is described which involves the selective release and enzymatic inactivation of non-microbial nucleotides, followed by the rapid and specific inhibition of the inactivating enzyme and the release and detection of microbial nucleotides in an appropriate assay, such as a bioluminescent assay.

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