Method of producing a composite preparation for making silage fr

Food or edible material: processes – compositions – and products – Fermentation processes – Of plant or plant derived material

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Details

426 53, 426 2, 426635, 426807, A23K 112, A23K 114

Patent

active

061327786

DESCRIPTION:

BRIEF SUMMARY
The present invention relates to a process for the preparation of a combination preparation which contains lactic acid-producing bacteria for ensiling green feed.
The use of preparations containing lactic acid-producing bacteria (LAB) and alkali metal salts of aliphatic fatty acids as ensiling agents for acidifying green feed and preventing aerobic degradation processes in the silage is known. DE-A-39 16 563 describes a combination preparation which contains alkali metal and/or alkaline earth metal formates, LAB and, if required, conventional carriers and/or assistants. The preparation can be used in the form of a scatterable mixture which is prepared, directly before addition to the material being converted into silage, by mixing the LAB in lyophilized (freeze-dried) form with the other components. Alternatively the bacteria and the formate may also be added separately to the material being converted into silage. The disadvantage of separate addition is that two operations have to be performed in ensiling.
DE-A-40 34 749 discloses, as an ensiling agent, a combination preparation which contains the LAB in an aqueous solution of alkali metal salts of aliphatic fatty acids. The preparation is present in the form of a suspension, which however must be used as rapidly as possible after preparation, since the LAB die within a short time. In the case of relatively long storage, it is therefore recommended that the aqueous solution of the alkali metal salt of the aliphatic fatty acid and the lyophilized bacterial culture be transferred separately to the user so that the components can be mixed shortly before use. However, a disadvantage of this procedure too is that the bacteria start to die immediately after the mixing procedure and, where there is a delay in ensiling, for example in the case of machine damage, rain or the like, the ensiling agent often can no longer be used. Another problem here is that, owing to the death of the bacteria after combination of the two components, the amount of bacteria still alive is frequently unknown, so that metering, in particular metering in the field, is extremely difficult.
It is an object of the present invention to provide a combination preparation for ensiling green feed, which preparation ensures better stability of the LAB.
We have found that this object is achieved and that, the stability of the LAB is exceptionally improved by mixing LAB with a carrier and then mixing this mixture with a salt of an aliphatic fatty acid. Unexpectedly, it was found that, in contrast to dissolution of the LAB in a sodium propionate or calcium propionate solution, as is known, in this case hardly any LAB die and, even on prolonged storage, the proportion of living bacteria is still extremely high.
The invention therefore relates to a process for the preparation of a combination preparation containing LAB, alkali metal and/or alkaline earth metal salts of aliphatic fatty acids and, if required, conventional assistants, wherein the LAB are mixed with a carrier to give a formulation and this formulation is mixed with at least one alkali metal and/or alkaline earth metal salt of an aliphatic fatty acid and, if required, conventional assistants. Preferred embodiments of the invention are defined in the following.
The LAB suitable for carrying out the novel process are not subject per se to any restriction. Strains of LAB which are suitable in principle are all those which grow under the conditions prevailing in the silo and produce sufficient lactic acid, ie. form lactic acid in an amount such that the resulting pH reduction permits no further development of microorganisms. Suitable LAB are those of the genus Lactobacillus, Lactococcus, Streptococcus, Staphylococcus or Pediococcus. Particularly good results were obtained with bacteria of the genus Lactobacillus plantarum. The bacteria are preferably used in lyophilized form.
Carriers which are suitable according to the invention are all carriers which are capable of adsorbing the LAB. Calcium carbonate or organic carriers, such as bran, pre

REFERENCES:
patent: 3677897 (1972-07-01), Jeffreys
patent: 4073889 (1978-02-01), Beck et al.
patent: 4789551 (1988-12-01), Sayle
patent: 4927763 (1990-05-01), Sudoma et al.

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