System useful for the production of proteins from recombinant DN

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4351723, 4352523, C12P 2100

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active

059424214

ABSTRACT:
A single celled organism, vector, and method are provided for continuous production of excreted proteins in the absence of substantial host cell replication and without the necessity for the addition of antibiotics to control cell replication. The vector has (1) an inducible promoter capable of activating a gene for a protein to be produced under conditions that substantially inhibit host cell replication, and (2) a hybrid gene containing a signal sequence fused to a gene for the protein to be produced. Large quantities of the transformed single celled organisms containing the vector can be grown in the absence of inducing conditions, thereby reducing the problem of plasmid loss. Further, the protein is produced under conditions that substantially inhibit host cell replication, thereby allowing immobilization of the single celled organism by entrapment or attachment within or onto a solid support surface. The single celled organism can be immobilized after, before or simultaneously with induction. The single celled organism advantageously may be an encapsulated gram-negative bacterium which has no outer membrane or an outer membrane that is permeable to the proteins to be produced so that the protein products are excreted into the media thereby avoiding difficulty in recovering the desired protein products due to the formation of inclusion bodies or the presence of contaminants produced by cell lyses.

REFERENCES:
patent: 4338397 (1982-07-01), Gilbert et al.
patent: 4356270 (1982-10-01), Itakura
patent: 4495287 (1985-01-01), Uhlin et al.
patent: 4595658 (1986-06-01), Zinder et al.
patent: 4624926 (1986-11-01), Inouye et al.
patent: 5747281 (1998-05-01), Shuler et al.
Brodelius et al., "Immobilized Plant Cells," Advances in Applied Microbiol., 28:1-26 (1982).
Klein et al., "Pore Size and Properties of Spherical Ca-Alginate Biocatalysts," Eur. J. Appl. Microbiol. Biotechnol., 18:86-91 (1983).
Pugsley et al., "Export and Secretion of Proteins by Bacteria," FEMS Microbiol. Rev., 32:3-38 (1985).
Lewin, Genes, John Wiley & Sons, New York, p. 680 (1983).
Lazzaroni et al., Production of Extracellular Alkaline Phosphatase By Escherichia coli K-12 Periplasmic-Leaky Mutants Carrying phoA+ Plasmids, Eur. J. Appl. Microbiol Biotechnol., 16:146-150 (1982).
Gilkes et al., "A Mutant of Escherichia coli That Leaks Cellulase Activity Encoded By Cellulase Genes From Cellulomonas Fimi," Biotechnology, 259-263 (1984).
Kato et al., "Extracellular Production of Bacillus penicillinase by Eschericha coli Carrying pEAP2," Eur. J. Appl. Microbiol. Biotechnol., 18:339-343 (1983).
Kudo et al., "Excretion of the Penicillinase of an * Alkalophilic Bacillus sp. Though the Escherichia coli Outer Membrane," J. Bacteriol., 156:949-951 (1983).
DeBoer et al., "Construction of a Tandem trp-lac Promotor and a Hybrid trp-lac Promoter for Efficient and Controlled Expression of the Human Growth Hormone Gene in Escherichia Coli, " In Promotors: Sturcture and Function, Rodriguez et al., (eds), Praeger Publishers, New York, 462-481 (1982).
Mossbach et al., "Formation of Proinsulin By Immobilized Bacillus subtilis," Nature, 302:543-545 (1983).
Shinmyo et al., "Physiology of alpha-amylase Production By Immobilized Bacillus amyloliquefaciens," Eur. J. Appl. Microbiol. Biotechnol., 14:7-12 (1982).
Dhulster et al., Culture and Bioconversion Use of Plasmid-Harboring Strain of Immobilized E. Coli, * Appl. Microbiol. Biotechnol., 20:87-93 (1984).
Younes et al., "Enhancement of Extracellular Enzymatic Activities Produced By Immobilized Growing Cells of Myxococcus xanthus," Appl. Microbiol. Biotechnol., 19:67-69 (1984).
Kokubu et al., "Protease Production By Immobilized Mycelia of Streptomyces fradiae, " Biotechnol. & Bioeng., 23:29-39 (1981).
Juarez et al., "Chromosomal Mutation That Affects Excretion of Hemolysin in Escherichia coli," J. Bacteriol., 159:1083-1085 (1984).
Atlan et al., "Optimized Extracellular Production of Alkaline Phosphatase by Iky Mutants of Escherichia coli K12," Appl. Microbiol. Biotechnol., 19:5-12 (1984).

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