Process for recovering protease

Details Process for recovering protease Process for recovering protease

1978-05-18

1980-07-15

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435213, 435218, 435219, 435225, 435 70, 435816, C12N 948, C07G 702

Patent

active

042129460

ABSTRACT:
Protease is recovered by reacting a first amino acid whose amino group is protected with a protective group with a second amino acid whose carboxyl group is protected with a protective group, in an aqueous medium in the presence of a protease to result a peptide synthesis to deposit the addition compound of a dipeptide and said second C-terminal protected amino acid; dissolving said addition compound by adding an organic solvent to a precipitate separated from the reaction mixture and isolating the protease from an organic solvent suspension or dissolving said addition compound by adding a water immiscible organic solvent and separating the organic solvent phase by a liquid-liquid separation.
The addition compound can be dissolved in an organic solvent after separating the precipitate from the reaction mixture.
The addition compound can be also dissolved by adding a water-immiscible organic solvent to the reaction mixture and the organic solvent phase can be separated from the aqueous phase.

REFERENCES:
patent: 4086136 (1978-04-01), Isowa et al.
patent: 4119493 (1978-10-01), Isowa et al.
Perlman, Methods in Enzymology, vol. XIX, pp. 646-647, 688-693, 696, 697, 758, 759 and 785 (1970).
Journal of Fermentation Technology 40, 346-353 (1962).
Journal of Biological Chemistry 212, 255-269 (1955).

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