Substrates for hydrolases

Details Substrates for hydrolases Substrates for hydrolases

1985-04-05

1987-12-29

Brown, J. R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 181, 536120, C07H 1702

Patent

active

047162228

ABSTRACT:
Chromogenic substrates of the formula A--O--B are described, in which A represents the radical in the ion A--O.sup.- of a compound A--OH, whose absorbance is measured photometrically after hydrolysis of A--O--B, while B is the radical in a compound B--OH which makes the compound specific for the reaction with a given enzyme, and where A--OH is a hydroxynitropyridine, a hydroxynitrobenzopyranone, a dibenzox(or thi)azine or a dibenzopyranone or a derivative thereof, and B is a sugar or sugar derivative, an amino acid or an oligopeptide or an inorganic acid.
These compounds can be used for the detection and determination of hydrolases.

REFERENCES:
patent: 2616889 (1952-11-01), Velluz et al.
patent: 3547828 (1970-12-01), Mansfield et al.
patent: 4038270 (1977-07-01), Higashiyama et al.

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