Composition, kit and method for assaying heparin and a method fo

Details Composition, kit and method for assaying heparin and a method fo Composition, kit and method for assaying heparin and a method fo

1989-03-14

1990-08-14

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

422 61, 436 69, 436 94, 436166, C12Q 156, G01N 2175, G01N 3386

Patent

active

049487248

ABSTRACT:
A composition, kit, and method for determining the concentration of heparin in a blood plasma sample and a method for making the composition. The method includes incubating a blood plasma sample with a known amount of Factor X.sub.a, combining the incubated product with an admixture of calcium chloride, brain phospholipids, and a buffered plasma fraction that has been produced by treating mammalian blood to substantially remove Factors II, VII, IX and X while retaining incidental amounts of Factor V and fibrinogen, and measuring the time it takes for clotting to occur after combining the incubated product with the admixture.

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Sigma Technical Bulletin No. 870, "A Clotting Procedure for the Quantitative Determination of Heparin in Plasma", Published by SIGMA Chemical Company, 1982.
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