Thermal technique for bulk fluid movement in capillary electroph

Details Thermal technique for bulk fluid movement in capillary electroph Thermal technique for bulk fluid movement in capillary electroph

1989-06-22

1990-03-06

Niebling, John F.

Plastic and nonmetallic article shaping or treating: processes

Forming continuous or indefinite length work

Shaping by extrusion

304299R, 3041801, 3041833, G01N 2726, B01D 5702

Patent

active

049063448

ABSTRACT:
Bulk fluid movement in a capillary electrophoresis system is achieved by imposing a temperature change on either a portion of the capillary or a bulb joined to the capillary at one end. The process may be used for sample loading, in which case a small volume introduction is made, or for flushing of the capillary with buffer solution, in which a larger volume introduction is made. The temperature change may be either an increase or a decrease, and the volume passed into or through the capillary is controlled to a high degree of precision by selection and accurate measurement of the volume of the bulb or capillary portion to which the temperature change is applied together with accurate control of the temperature change itself.

REFERENCES:
patent: 3932264 (1976-01-01), Haruki et al.
patent: 4676897 (1987-06-01), Kuze et al.
Smith, R. D. et al. "Sample introduction and separation in capillary electrophoresis and combination with mass spectrometric detection" Talanta, 36(1-2) pp. 161-169 (1989).
Verheggen, T. P. et al. "Simple sampling device for capillary isotachophoresis and capillary zone electrophoresis" Journal of Chromatography, 452, pp. 615-622 (1988).
Rose, D. J. et al. "Characterization and automation of sample introduction methods for capillary zone electrophoresis" Analytical Chemistry, 60(7), pp. 642-8 (1988).
Huang, X. et al. "Bias in quantitative capillary zone electrophoresis caused by electrokinetic sample injection" Analytical Chemistry 60(4) pp. 375-377 (1988).
Honda, S. et al "Evaluation of an automatic siphonic sampler for capillary zone electrophoresis" Journal of Chromatography, 404(2), pp. 313-320 (1987).
Wallingford, R. A. et al. "Characterization of a microinjector for capillary zone electrophoresis" Analytical Chemistry, 59(4), pp. 678-681 (1987).
Lukacs, K. D. et al. "Capillary zone electrophoresis: effect of physical parameter on separation efficiency and quantitation" J. High Resolution. Chromatogr. Chromatogr. Commun. 8(8) pp. 407-411 (1985).

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