Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1983-12-23
1987-06-30
Hazel, Blondel
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435240, 935 94, C12N 1500, C12N 500
Patent
active
046770666
ABSTRACT:
A method for promoting fusion of plant protoplasts comprising the steps of; (a) treating the plant protoplasts with a fusion agent, and then (b) incubating the resultant plant protoplasts in an incubation medium having an osmotic pressure lower than that required for maintaining the plant protoplasts in a stable form. This effectively promotes the fusion of the plant protoplasts at a higher fusion rate in a wider range of plants.
REFERENCES:
Miller et al., 1973, "Plant Protoplast Culture", in Tissue Culture Methods and Applications, Academic Press, pp. 500-505.
Gamborg, 1976, "Somatic Cell Hybridization by Protoplast Fusion and Morphogenesis", in Plant Tissue Culture & Its Biotechnological Application, pp. 287-301.
Eriksson, 1976, "Technical Advances in Protoplast Isolation and Culture", in Plant Tissue Culture and its Biotechnological Application, pp. 316-322.
K. K. Kartha et al., MPC, Ltd. Iwakuni, Can. J. Bot., vol. 52, 1974, "Fusion of Rapeseed and Soybean Protoplasts and Subsequent Division of Heterokaryonytes", pp. 2-5.
F. Constabel et al., MPC, Ltd. Iwankuni, Z. Pflanzenphysiol. Bd. 79. S. 1-7., 1976, "Cell Division of Intergeneric Protoplast Fusion Products", pp. 6-12.
K. N. Kao and M. R. Michayluk, Planta (Berl.) 115, 355-367, (1974), "A Method For High--Frequency Intergeneric Fusion of Plant Protoplasts".
K. N. Kao, F. Constabel, M. R. Michayluk and O. L. Gamborg, Planta (Berl.) 129, 215-227, (1974), "Plant Protoplast Fusion and Growth of Intergeneric Hybrid Cells".
"The Expression of Tumour Markers in Intraspecific Somatic Hybrids of Normal and Crown Gall Cells from Nicotiana Tabacum", by Wullems et al., Theor. Appln. Genet. 56, 203-208, (1980).
Maeda Yasuhiro
Takahashi Shigeru
Fox David T.
Hazel Blondel
Mitsui Petrochemical Industries Ltd.
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