Process for the purification of DNA on diatomaceous earth

Details Process for the purification of DNA on diatomaceous earth Process for the purification of DNA on diatomaceous earth

1990-12-18

1991-12-24

Rollins, John W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 28, 536 29, 536127, 435803, 423335, C07H 2100, C07H 2104

Patent

active

050754300

ABSTRACT:
This invention is directed to a process for the purification of plasmid and other DNA, both single-stranded and double-stranded, by immobilizing the DNA onto diatomaceous earth in the presence of a chaotropic agent and eluting the DNA with water or low salt buffer. The resulting purified DNA is biologically active. Also included in the invention is a process for the immobilization of DNA onto diatomaceous earth in the presence of a chaotropic agent.

REFERENCES:
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patent: 4923978 (1990-05-01), McCormick
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N. S'yakste et al. Analysis of Cellular Nucleoprotein with Nucleo-Protein-Celite Chromatography. 1986 Plenum Publishing Corp. pp. 1007-1016.
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