DNA probes specific for virulent listeria monocytogenes

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 536 243, 536 2433, 536 266, C12Q 168, C12P 1934, C07H 2104, C07H 1904

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active

056100124

ABSTRACT:
An assay for detecting virulent L. monocytogenes is provided. This assay includes the steps of: contacting the nucleic acids of L. monocytogenes with a probe under conditions permitting hybridization; and detecting any probe that hybridizes to the nucleic acids. The probe used in this method includes a DNA sequence selected from a group consisting of a 0.9 kb HindIII-EcoRI fragment of plasmid pLUCH52, or a part thereof; a 1.1 kb HindIII-EcoRI fragment of plasmid pLUCH51, or a part thereof; and a 1.8 kb HindIII-EcoRI fragment of plasmid pLUCH44, or a part thereof).

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